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UMICH Bru-seq Lab

Welcome to the UMICH Bru-seq Lab Core!

What information can I find on this page?

  • Overview
    • Our services 
    • What steps should I follow to submit a sample?
    • Sample drop-off
    • Mailing samples
  • Leadership
  • Location and hours of operation
  • Protocols
  • Publications
  • Contacts

 

Overview

The University of Michigan Bru-seq Lab is a core facility that provides nascent RNA sequencing services for samples prepared using the Bru-seq suite of techniques. We serve researchers from the University of Michigan, and other institutions in the United States and around the worldUsers can request services for these four assays:

  • Bru-seq: To measure synthesis of RNA
  • BruChase-seq: To measure stability of RNA
  • BruUV-seq: To identify transcription start sites and capture unstable transcripts such as enhancer RNA and PROMPTs
  • BruDRB-seq: To measure RNA Polymerse II elongation rates via RNA

 

 Our services

  • Total RNA and nascent Bru-RNA isolation
  • cDNA library preparation
  • Paired-end 150bp Next-Generation Sequencing (using the University of Michigan Advanced Genomics Core)
  • NGS sequencing data processing
  • Data storage 

 

What steps should I follow to submit a sample?  

 

Please refer to the Protocols section for information regarding preparation of samples for the four Bru-seq assays.

  1. Create an “Experimental Plan” request under the Request Services tab.
  2. Once you submit the form, we will process it on our end and reach out to you if we have any questions.
  3. Once the “Experimental Plan” form has been approved, please submit a  “Bru-seq Sample Submission Request” under the Request Services tab.
  4. Once the “Bru-seq Sample Submission Request” has been approved, please either edit or schedule a sample Drop-Off, under the Schedule Drop-Off tab. Please go to "View Schedule" to reserve your time. If you are mailing us your samples, you can skip this step.
  5. Once we receive your samples, they get put in a queue. We are unable to commit to a turnaround time as the processing time is usually highly variable, but will remain in contact with you through iLabs with updates. 

 

Sample drop-off:

 

Sample drop-off must be prearranged. Please refer to the "Location and hours of operation" section before scheduling a drop-off.

 

Please submit samples in TRIzol unless prior permission has been obtained.

Exception: Samples can be submitted as cells in culture (plates, flasks) only by UofM NCRC occupants. 

 

Mailing samples:

 

Samples must be submitted in TRIzol.

 

Please ship your samples on dry ice to this address:

Michelle Paulsen

1410 Bldg 520, NCRC

1600 Huron Parkway

Ann Arbor, MI-48109

734-763-0448

 

 

 

Leadership

Mats Ljungman | Core Director

Michelle Paulsen | Core Manager

Location and hours of operation

Hours Location

Monday - Friday

7am - 4pm

Sample submission must be prearranged              

1410 B520 NCRC

1600 Huron Parkway

Ann Arbor, MI  48109

 Protocols

 Publications

Contacts

Name Role Phone Email Location
Michelle Paulsen
Core Manager
 
734-763-0448
 
tenbroek@umich.edu
 
1351 B520 NCRC
 
Mats Ljungman
Core Director
 
734-674-3330
 
ljungman@umich.edu
 
1346 B520 NCRC
 
Ishwarya Venkata Narayanan
Data Analysis
 
734-763-0448
 
ivenkat@umich.edu
 
1356 B520 NCRC