UMICH Microscopy, Imaging and Cellular Physiology Core (MICPC)

Overview of Services

Microscopy, Imaging, and Cellular Physiology Core (MICP Core) of the Michigan Diabetes Research Center (MDRC).  The MICP Core provides state-of-the-art protein and RNA imaging and cell physiological analysis to enhance the diabetes-related research at the University of Michigan. The MICP Core provides expertise, advice, training and equipment for microscopic and morphometric analysis of both protein and RNA in biologic samples in support of research focused on diabetes and its complications or related endocrine and metabolic disorders. The MICP Core provides access to a wide variety of microscopy, imaging and electrophysiology equipment that otherwise would be beyond the scope and resources of most investigators. The overwhelming majority of MDR Core investigators who require such techniques rely on the MICP Core to provide them and their staff with expert consultation, training and support for routine and advanced microscopy and image analysis for protein and RNA morphometric studies and for optogenetic and electrophysiology studies.

Services:

Imaging Laboratory
• Consultation and Training for Widefield and Confocal Microscopy
• Access to Widefield and Confocal Microscopes for Fixed and Live Cells and Tissues
• Consultation and Training for Image Analysis
• Access to Windows PC Workstations for Image Analysis

In Situ Hybridization Laboratory
• Consultation
• Probe labeling by in vitro transcription
• Tissue processing
• Complete ISH procedure (hybridization and signal detection)
• Dual label ISH / immunohistochemistry
• Data analysis

Equipment and Technology:

Imaging Laboratory
• Nikon Inverted TiE Widefield and A1 Confocal Microscope
• Leica SP5 Inverted Confocal Microscope
• Olympus Fluoview 500 Inverted Confocal Microscope
• Windows PC Workstations for Image Analysis
     Image Analysis Software: 3D Imaris, MetaMorph, AutoQuant deconvolution, 3D Volocity, MATLAB, ImageJ, Offline Nikon Elements, Offline Leica Application Suite (LAS X)

In Situ Hybridization Laboratory
• Radioisotopes: Autoradiographic Film and Emulsion
• Fluorescence: RNAprobe using RNAscope Technology
• Dual Label In Situ Hybridization and Immunohistochemistry

Leadership

Michigan Diabetes Research Center (MDRC)
Martin G. Myers, Jr., MD, PhD MRDC Director mgmyers@umich.edu
Mary A. Malec MDRC Director of Administration 734-764-6103 mmalec@umich.edu

Microscopy, Imaging and Cellular Physiology Core (MICP Core)
David A. Antonetti, PhD The MICP Core Director 734-232-8230 dantonet@med.umich.edu

Imaging Laboratory
Stephen I. Lentz, PhD Imaging Laboratory Director 734-647-8233 lentzs@umich.edu

In Situ Hybridization Laboratory
Carol F. Elias, PhD In Situ Hybridization Laboratory Director 734-647-2801 cfelias@umich.edu


Location and hours of operation 

Hours:   Location:      
Imaging Laboratory:           
Monday - Friday  9 am - 5 pm 1000 Wall St.  Brehm Tower, Room 6245 Ann Arbor, MI 48105-5714
           
In Situ Hybridization Laboratory:      
Monday - Friday 9 am - 5 pm 1137 Catherine St. Med Sci II Room 7725 Ann Arbor, MI 48109

 

Links and Resources

  1. MDRC
  2. MDRC Cores
  3. MICP Core
  4. MICP Core: Imaging Laboratory
  5. MICP Core: In Situ Hybridization Laboratory

Contacts

Name Role Phone Email Location
Imaging Laboratory

 

 

 

 
Stephen I. Lentz, PhD
Imaging Laboratory Director
 
734-647-8233
 
lentzs@umich.edu
 
Brehm Tower, Room 6245
 
In Situ Hybridization Laboratory

 

 

 

 
Carol F. Elias, PhD
In Situ Hybridization Laboratory Director
 
734-647-2801
 
cfelias@umich.edu
 
Med Sci II, Room 7732B
 
David Garcia-Galiano
In Situ Hybridization Manager
 
734-647-3875
 
dgarciag@umich.edu
 
Med Sci II Room 7725
 
Emily Henson
Assistant to the manager
 
734-647-3875
 
hensone@umich.edu
 
Med Sci II Room 7725
 
Susan Allen
Assistant to the manager
 
734-647-3875
 
sjallen@umich.edu
 
Med Sci II Room 7725
 

Available Equipment and Resources

Name Price
Nikon A1 Microscope View calendar
Leica SP5 Confocal Microscope View calendar
Olympus Fluoview 500 Confocal Microscope View calendar
Windows-PC Workstation #1 for Image Analysis View calendar
Windows-PC Workstation #2 for Image Analysis View calendar
Windows-PC Workstation #3 for Image Analysis View calendar