UMICH Microscopy and Imaging Analysis Core (MIAC) Michigan Diabetes Research Center

Overview of Services

Microscopy and Image Analysis Core (MIAC) of the Michigan Diabetes Research Center (MDRC).  The MIAC provides state-of-the-art protein and RNA imaging and cell physiological analysis to enhance the diabetes-related research at the University of Michigan. The MIAC provides expertise, advice, training and equipment for microscopic and morphometric analysis of both protein and RNA in biologic samples in support of research focused on diabetes and its complications or related endocrine and metabolic disorders. The MIAC provides access to a wide variety of microscopy and imaging equipment that otherwise would be beyond the scope and resources of most investigators. The overwhelming majority of MDR Core investigators who require such techniques rely on the MIAC to provide them and their staff with expert consultation, training and support for routine and advanced microscopy and image analysis for protein and RNA morphometric studies.

Services:

Imaging Laboratory
• Consultation and Training for Widefield and Confocal Microscopy
• Access to Widefield and Confocal Microscopes for Fixed and Live Cells and Tissues
• Consultation and Training for Image Analysis
• Access to Windows PC Workstations for Image Analysis

In Situ Hybridization Laboratory
• Consultation
• Probe labeling by in vitro transcription
• Tissue processing
• Complete ISH procedure (hybridization and signal detection)
• Dual label ISH / immunohistochemistry
• Data analysis

Equipment and Technology:

Imaging Laboratory
• Leica STELLARIS 8 FALCON Inverted Confocal Microscope
• Nikon Inverted TiE Widefield and A1 Confocal Microscope
• Leica SP5 Inverted Confocal Microscope
• Windows PC Workstations for Image Analysis
     Image Analysis Software: AI-based Aivia, 3D Imaris, MetaMorph, AutoQuant deconvolution, 3D Volocity, MATLAB, ImageJ, Offline Nikon Elements, Offline Leica Application Suite (LAS X)

In Situ Hybridization Laboratory
• Radioisotopes: Autoradiographic Film and Emulsion
• Fluorescence: RNAprobe using RNAscope Technology
• Dual Label In Situ Hybridization and Immunohistochemistry

Leadership

Michigan Diabetes Research Center (MDRC)
Martin G. Myers, Jr., MD, PhD MDRC Director mgmyers@umich.edu
Mary A. Malec MDRC Director of Administration 734-764-6103 mmalec@umich.edu

Microscopy and Image Analysis Core (MIAC)
David A. Antonetti, PhD The MIAC Director 734-232-8230 dantonet@med.umich.edu

Imaging Laboratory
Stephen I. Lentz, PhD Imaging Laboratory Director 734-647-8233 lentzs@umich.edu

In Situ Hybridization Laboratory
Carol F. Elias, PhD In Situ Hybridization Laboratory Director 734-647-2801 cfelias@umich.edu

 

Please remember to cite the Michigan Diabetes Research Center (MDRC) in publications as follows:
“The project described was supported by Grant Number P30DK020572 (MDRC) from the National Institute of Diabetes and Digestive and Kidney Diseases”. 

The MIAC encourages you to include how the Imaging or In Situ Hybridization Labs supported your research.

 

Location and hours of operation 

Hours:   Location:      
Imaging Laboratory:           
Monday - Friday  9 am - 5 pm 1000 Wall St.  Brehm Tower, Room 6245 Ann Arbor, MI 48105-5714
           
In Situ Hybridization Laboratory:      
Monday - Friday 9 am - 5 pm 2800 Plymouth Rd

NCRC B025  3620

Ann Arbor, MI 48109

 

Links and Resources

  1. Caswell Diabetes Institute
  2. Michigan Diabetes Research Center (MDRC)
  3. MDRC Cores
  4. MDRC Microscopy and Image Analysis Core (MIAC)
  5. MDRC - MICP Core: Imaging Laboratory
  6. MDRC - MICP Core: In Situ Hybridization Laboratory

Contacts

Name Role Phone Email Location
Diabetes Center: Stephen Lentz PhD and Carol Elias, PhD
Microscopy and Image Analysis Core (MIAC)
 
734-647-8233 / 734-647-2801
 
lentzs@umich.edu / cfelias@umich.edu
 
Brehm Tower, Room 6245
 
Imaging Laboratory

 

 

 

 
Stephen I. Lentz, PhD
Imaging Laboratory Director
 
734-647-8233
 
lentzs@umich.edu
 
Brehm Tower, Room 6245
 
In Situ Hybridization Laboratory

 

 

 

 
Carol F. Elias, PhD
In Situ Hybridization Laboratory Director
 
734-647-2801
 
cfelias@umich.edu
 
MNCRC B025 3620
 
Emily Henson
Assistant to the manager
 
734-647-3875
 
hensone@umich.edu
 
NCRC B025 3620
 
Susan Allen
Assistant to the manager
 
734-647-3875
 
sjallen@umich.edu
 
NCRC B025 3620
 

Available Equipment and Resources

Name Price
Leica STELLARIS 8 FALCON Confocal Microscope View calendar
Nikon A1 Microscope View calendar
Leica SP5 Confocal Microscope View calendar
Windows-PC Workstation #1 for Image Analysis View calendar
Windows-PC Workstation #2 for Image Analysis View calendar
Windows-PC Workstation #3 for Image Analysis View calendar
Windows-PC Workstation #4 for Image Analysis View calendar
VRC Dell Precision 5820 Offline Image Analysis View calendar
VRC 16-Core ASUS Offline Image Analysis View calendar
Olympus Fluoview 500 Confocal Microscope View calendar
Leica Mica - Hands-On Workshop View calendar