UMICH Proteomics Resource Facility

Overview of Services

Mass Spectrometry-Based Proteomics Resource Facility

Department of Pathology, University of Michigan

The mass spectrometry-based Proteomics Resource Facility (PRF) at the Department of Pathology began its operations in 2007 and the facility occupies about 1600 sq feet of space in Room 3554/3556 at the MSRB-2 Building. 

The goal of the PRF is to assist researchers in designing proteomic experiment, maintain state-of-the-art instrumentation, provide technical expertise and bioinformatic support during both discovery and validation phases of proteomic experiments. 

Services offered by the PRF include

• Identification of proteins following in-gel (Coomassie and Silver-stained gels), in-solution, and on-bead digestions.
• Shot-gun proteomic analysis of complex mixtures using either one- or two-dimensional liquid chromatography (2D-LC).
• Identification of various post-translational modifications (ex. Phosphorylation, Acetylation, Ubiquitylation, Methylation etc).
• Relative quantitation (differential proteomic analysis) using either isotope labeled ( ex. SILAC, TMT etc) or label-free spectral counting methods.
• Targeted proteomics using Parallel Reaction Monitoring (PRM).
• Many more....

The PRF is equipped with two Thermo Scientific mass spectrometers devoted exclusively for proteomic analysis.  These include:

1. Orbitrap Fusion Tribrid with ETD 
2. Q Exactive HF

Both mass spectrometers are equipped with dedicated nano-LC systems (Dionex RSLC-nano).

PRF is now a Shared Resource Facility for the following Centers.

• Rogel Cancer Center:  There is a 30% subsidy on ALL services, maximum $6,000/PI/year to Rogel CC members
• UM Center for Gastrointestinal Research (UMCGR):  There is a 100% subsidy on all services up to $1500/PI/Fiscal Year for all members of UMCGR.

Campus/Shipping Address

Attn: Venkatesha Basrur, Ph.D.

MSRB-2, Rm #3554/3556
1150 W. Medical Center Dr
Ann Arbor, MI 48109

Acknowledgment of Contributions

Publications containing data generated by the Proteomics Resource Facility (PRF) should include an acknowledgement of the PRF or include members as coauthors.  The PRF needs these acknowledgements and authorship to help demonstrate our contributions to the research community.  This in turn helps secure future funding to maintain a robust Core facility and provide professional development of its staff.  The Association of Biomolecular Resource Facilities (ABRF) has published a guideline to use when considering whether or not to include core laboratory members on publications.

Leadership

Alexey Nesvizhskii, Ph.D | Department of Pathology, Department of Computational Medicine and Bioinformatics

Venkatesha Basrur, Ph.D | Department of Pathology

Location and hours of operation

Links and Resources

• Proteomics Resource Facility
• Waiver & Disclaimer Form

Contacts