UMICH CyTOF Core

Overview of Services

The CyTOF Core at the University of Michigan was founded in 2016 to promote a collaborative and interdisciplinary environment across the University of Michigan campus. Our goal is to share our equipment and expertise with ambitious researchers to help solve the most challenging problems in cellular biology, immunology, oncology, and medicine.

Instruments:  

• Helios CyTOF System (Suspension CyTOF)
  
• Hyperion CyTOF System (Imaging CyTOF)
  
• Attune Flow Cytometer
• ÄKTA Pure FPLC
• NanoDrop 2000c Spectrophotometer

Selected Services: (Please login for a full list of services) 

• Helios and Hyperion antibody panels for both human and mouse samples
  
• Custom CyTOF Antibody Conjugation
• Data acquisition by Mass Cytometry (Helios)
• Data acquisition by Imaging Mass Cytometry (Hyperion)
• CyTOF consultation
• Core Rogel Cancer Center members receive a discount on all CyTOF services (see CyTOF Core User Guidelines for details)

Antibody Panels Available:

• Mouse suspension cells (17-Ab): EpCAM, CD45, Gr-1, CD11b, CD11c, CD49b(DX5), F4/80, CD103, CD169, CD206, CD45R (B220), CD3, CD4, CD8, IA-IE, CD86, CD80
• Mouse suspension cells (33-Ab): EpCAM, CD45, Gr-1, CD11b, CD11c, CD49b (DX5), F4/80, CD103, CD169, CD206, CD45R (B220), CD3, CD4, CD8, CD69, CD25, CD44, CD62L, CD127, CXCR5, PD-1, IgM, IgG, IgD, IgA, IgG1, FAS (CD95), GL7, CD38, CD138, IA-IE, CD86, CD80
• Mouse suspension cells with cytokines (35-Ab): CD45, Ly-6G, Ly-6C, Siglec-F, CD206, CD103, CD49b, CD49d, CD11c, CD11b, CD24, KLRG1, CD64, B220, CD3, CD4, CD8, CD19, CD25, TCRγδ, CXCR5, CD127, CD62L, CD44, CD69, IgD, IgM, TNFalpha, IFNg, IL-10, IL-4, Granzyme-B, Ki67, IA-IE, mPDCA-1
• Mouse FFPE tissue Immune: CD11b, F4/80, Ly-6G, CD45R (B220), CD4, CD8, IA-IE, CD31, Collagen IV
• Mouse FFPE tissue Cancer: Beta-actin, Beta-catenin, BRCA1, E-Cadherin, EpCAM, Vimentin, Ki-67, CD31, Collagen IV
• Mouse frozen tissue: CD45, Ly-6G, Ly-6C, CD206, CD11c, CD11b, CD24, CD64, CD45R(B220, CD3, CD4, CD8, CD25, CXCR5, CD127, CD44, IA-IE, mPDCA-1 (CD317), CD31, EpCAM, Collagen IV
• Human suspension cells Immune: CD45, CD66b, CD11c, CD16, CD123, CD161, CD14, CD56, CD294, CD19, CD20, CD3, CD4, CD8, TCRgd, CCR7, CD45RA, CD45RO, CD57, CD127, CD25, CCR6, CXCR3, CCR4, CXCR5, CD38, CD27, IgD, HLA-DR, CD28
• Human FFPE tissue, Cancer: EpCAM, Pan-Cytokeratin, ALDH1A1, CD44, Progesterone receptor, CD24, HER2, Vimentin, ALDH1A3, Pan-Actin, Estrogen Receptor alpha, Ki-67, CD45
• Human FFPE tissue, Immune: Pan-Actin, EpCAM, Ki-67, CD31, CD11c, CD14, CD15, CD16, CD68, CD20, CD4, CD8, HLA-DR 

New Users

• If you are not registered with iLabs, please contact your PI and register
• Please review the CyTOF Core User Guidelines
  

• After registering with iLabs and reviewing the user guidelines, please send a completed user registration form to trhoang@umich.edu
  

Location and hours of operation

Links and Resources

• Fluidigm products
• Recent Helios Publications
• Recent Hyperion Publications
• CyTOF Core User Guidelines
• CyTOF Core User Registration Form
• Available antibody panels and prices
• Protocols (Note that these protocols will likely require optimization)
• Single Cell Preparation Guide (Helios)
• Maxpar Cell Surface Staining Protocol (Helios)
• Imaging Mass Cytometry Staining Protocol for FFPE Sections (Hyperion)
• Data analysis (single cell data)
• Cytobank (viSNE, SPADE)
• FlowJo
• A Beginner’s Guide to Analyzing and Visualizing Mass Cytometry Data
• Data Analysis (tissue sections)
• MCD Viewer
• histoCAT
• Cell Profiler

Contacts