UMICH Transgenic Animal Model Core

Overview of Services

The University of Michigan Transgenic Animal Model Core was established in 1989 in response to the need for transgenic technology by University Investigators. The mission of the Transgenic Core is to provide access to transgenic animal technology in an efficient, effective manner. This includes:

  1. Production of transgenic mice
  2. CRISPR/Cas9 mediated genome editing in mice and rats
  3. Production of transgenic rats and zinc finger nuclease gene knockout and knockin rats
  4. Production of gene-targeted mouse embryonic stem (ES) cells for mouse knockout models and in vitro studies
  5. Production of ES cell-mouse chimeras for mouse knockout models
  6. Assisted reproduction technology for mice and rats
  7. De novo derivation of mouse ES cells from custom mice
  8. Hands-on training in mouse ES cell manipulation, pronuclear microinjection, and blastocyst injection

Researchers can use Transgenic Core laboratory space and resources side by side with staff members. Consultation in all phases of transgenic and gene targeting research from experimental design to mouse breeding is freely available. Meet the Staff.


The Transgenic Core collaborates with the BAC Recombineering Core to prepare genetically modified bacterial artificial chromosomes (BACs). The mission of the BAC Core is to provide access to a complex technology to investigators so that they can use their resources to conduct research instead of developing tools for research. BACs are genetically modified by homologous recombination (BAC recombineering) to prepare DNA mutations, insertions and deletions. BACs can be used as transgenes to express reporter molecules (GFP), Cre recombinase, and mutant alleles in cell culture and mice. The BAC Core also prepares gene targeting vectors for the modification of chromosomes in ES cells.


The Transgenic Mouse Core guarantees that at least three transgenic founder mice will be produced for each DNA construct. The overall average is ten founders. Since 1989 over 15,000 mouse transgenic founders were produced from more than 1,500 plasmid and BAC transgenes. Nearly 1000 transgenic rat founders have been produced, including BAC transgenic rat strains. Over 250 new mutant mouse models were produced by the electroporation of mouse ES cells with targeting vectors and another 60-odd strains were produced from ES cell clones obtained from the international mouse knock out project (KOMP). Some of these mouse and rat models have been published.

 

The Transgenic Core has used CRISPR/Cas9 technology to target genes in both mice and rats.  We provide testing of CRISPR/Cas9 reagents in both blastocysts and mouse ES cells or rat embryonic fibroblasts to confirm activity before using them to generate animal models.  We will work with investigators in design and development of CRISPR/Cas9 reagents specific to the investigator's project.

 

Leadership and Core Staff

Name Role Phone Email Location FAX
Thom Saunders Director 734-647-2910 tsaunder@umich.edu 2570 MSRB II SPC 5674 734-936-2622
Elizabeth Hughes Assistant Director 734-647-9248 elidale@umich.edu 2578 MSRB II SPC 5674 734-936-2622
Wanda Filipiak Staff 734-763-6209 wandaf@umich.edu 2526 MSRB I SPC 5674 734-615-1402
Galina Gavrilina Staff 734-763-6209 galinag@umich.edu 2526 MSRB I SPC 5674 734-615-1402
Anna LaForest Staff 734-763-6209 annalaf@umich.edu 2526 MSRB I SPC 5674 734-615-1402
Michael Zeidler Staff 734-764-3666 zeidler@umich.edu 2574 MSRB II SPC 5674 734-936-2622
Corey Ziebell Staff 734-764-0781 ckibit@umich.edu 2574 MSRB II SPC 5674 734-936-2622

 

Location and Hours of Operation

Hours: Monday - Friday 8:00 AM - 5:00 PM

Location: 2570 MSRB II SPC 5674

                1150 W. Med. Center Dr.

                Ann Arbor, MI 48109

 

Links and Resources

http://www.med.umich.edu/tamc

http://www.med.umich.edu/tamc/service.html

Contacts

Name Role Phone Email Location
Thom Saunders
Director
 
734-647-2910
 
tsaunder@umich.edu
 
2570 MSRB II SPC 5674
 
Elizabeth Hughes
Assistant Director
 
734-647-9248
 
elidale@umich.edu
 
2578 MSRB II SPC 5674